Hi
everyone! I would like to share about what I have learnt on Tuesday.
I learnt about the MICROBIAL GROWTH. Things that I want to highlight
are the physical requirements and the chemical requirements. The
physical requirements are include light, pH, temperature, water
activity and osmotic pressure. The chemical requirements are water,
carbon, oxygen, nitrogen, phosphorus and sulphur, special growth
factor (ferum) and trace elements (Mg).
On
Thursday, I learnt about isolation, maintaining and preserving
microbes. There are 2 basic culture techniques used in microbiology
which are liquid culture and culture plates. The objectives of
culturing bacteria is to obtain pure culture and to use suitable
medium. Some of the bacteria grow by budding, spores and
fragmentations.
The
bacterial growth curve consists of lag phase, log phase, stationary
phase and death phase. For direct measurements of microbial growth,
we might have to count the bacteria by plate counts, filtration or
direct microscopic count. Lastly, we have to transfer the strains to
fresh medium then incubate and store in the refrigerator
(short-term), deep-freezer or freeze dryer.
*We went to ICOE talk on Monday. The talk is about Biotechnology and it was awesome! Thanks to Dr. Wan for bringing us :)
